TR-ISE Immunostain Enhancer Kit
Two reagents that make a weak stain readable: a blocking buffer for the slide, an antibody diluent for the antibody. Signal typically rises 3–10× while background falls.
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One Kit, Two Bottles, Two Jobs
TR-ISE-B handles the blocking step on the slide. TR-ISE-D is the medium your primary and secondary antibodies are diluted in.
Immunofluorescence Blocking Buffer
TR-ISE-B
The blocking step before the primary antibody. Raise serum from the secondary-antibody host animal to 2%, apply to the slide and incubate for 30 minutes.
- Blocking step for tissue sections
- 2% serum from the secondary-antibody host animal
- 30-minute incubation on the slide
- Lowers non-specific binding and background
Antibody Diluent
TR-ISE-D
The medium for primary and secondary antibodies. Dilute in TR-ISE-D, then incubate as in any standard immunostaining protocol.
- Dilution medium for primary and secondary antibodies
- Signal increase typically 3–10× in IHC and IF
- Improves antibody penetration into tissue
- Standard immunostaining incubation afterwards
Signal, Background, Antibody Volume
Where TR-ISE Helps Most
Weak IHC Signals
For slides where 4% formaldehyde fixation left antigen exposure poor and the stain came out flat.
Multiplex Immunofluorescence
Higher signal and lower autofluorescence background across every channel of a multiplex panel.
Immunocytochemistry
The same enhancer protocol for cell-based assays and cytospin preparations.
Deep Tissue Antigens
Improved penetration for antigens that ordinary antibody dilution struggles to reach.
Low-Input Samples
Detects weak targets without re-cutting the block or re-collecting the specimen.
Cost-Conscious Panels
Reaches optimal detection with less antibody per target — the volume you buy goes further.
Works with the rest of the TerryDR stack
TR-Dewax-AR all-in-one pre-treatment
Deparaffinization, hydration and antigen retrieval in one 30-minute run — before the blocking step.
TR-ASR antibody strip reagent
The strip step that clears bound antibodies between rounds of cyclic multiplex staining.
TR-Stainer automated IHC stainer
Microfluidic-chip-controlled incubation that runs standard primary and secondary antibodies.
TerryDR spatial analysis software
Nine QuPath modules — preprocessing, registration, segmentation, phenotyping, and spatial statistics.
Get more signal from the antibody you already buy
We'll send the protocol, the full feature list, and pricing for both bottles.